Static Droplet Array Cultivation Technology

The device is equipped with a static droplet array chip that houses tens of thousands of cultivation microchambers, each with a picoliter-scale volume. When a sample suspension is introduced into the chip, individual cells are gently distributed into the microchambers according to Poisson distribution, This is followed by mass parallelled cultivation, bright-field imaging for cellular growth and multiplication, fluorescence imaging (when necessary), A.I. based selection of targeted clones and their individualized non-contact exportation, all at single-cell resolution and for each of the selected microchambers.

AI-Assisted Image Processing Technology

The device integrates an autofocus imaging system capable of capturing high-resolution images for each microchamber. Using advanced image processing algorithms, it automatically analyzes individual clones within the chambers and intelligently identifies the desired target chambers. All imaging data and the spatial coordinates of the clones are automatically recorded and stored for subsequent traceability and data analysis.

Non-Contact Optical Manipulation of Microdroplets

Built on a high-precision automated platform, the device allows for precise localization of target microscopic clones. Using optical manipulation technology, it remotely captures these clones and encapsulates them in microdroplets for efficient recovery. The entire clone picking process is non-contact, significantly reducing the risk of contamination.