pDEP-RACS (Positive Dielectrophoresis-induced Deterministic Lateral Displacement-based Raman-Activated Cell Sorting) is an advanced method for isolating and analyzing single cells, particularly useful in microbiology and biomedical research.
Key Features:
-Integrated Technology: pDEP-DLD-RACS combines positive dielectrophoresis (pDEP), deterministic lateral displacement (DLD), and Raman-Activated Cell Sorting (RACS) for high-throughput, label-free cell screening and sorting based on metabolic phenotypes.
-pDEP: Manipulates cells based on their dielectric properties, driving them towards specific microfluidic channels.
-DLD: Uses microstructures to filter and sort cells by size, enabling physical separation of cells within a microfluidic device.
-RACS: Analyzes the molecular composition of cells using Raman spectroscopy, allowing identification and sorting based on unique molecular fingerprints.
Advantages:
-High-Throughput: Capable of processing cells at a high rate, suitable for large-scale studies and clinical applications.
-Label-Free Analysis: Does not require fluorescent labels or markers, preserving the natural state of cells.
-Precision Sorting: Sorts cells based on size, metabolic activity, and molecular composition.
pDEP-RACS represents a convergence of microfluidics, dielectrophoresis, and Raman spectroscopy, offering a powerful tool for cellular analysis with broad implications for biological research and clinical diagnostics.
References:
1. Xixian Wang#, Lihui Ren#, Zhidian Diao#, Yuehui He#, Jiaping Zhang, Min Liu, Yuandong Li, Lijun Sun, Rongze Chen, Yuetong Ji, Jian Xu*, and Bo Ma*. Robust spontaneous Raman flow cytometry for single-cell metabolic phenome profiling via pDEP-DLD-RFC. Advanced Science, 2023, 10(16): e2207497.
2. Xixian Wang#, Yi Xin#, Lihui Ren#, Zheng Sun, Pengfei Zhu, Yuetong Ji, Chunyu Li, Jian Xu*, Bo Ma*, Positive dielectrophoresis based Raman-activated droplet sorting for culture-free and label-free screening of enzyme function in vivo. Science Advances, 2020, 6(32): eabb3521
3. Xixian Wang#, Lihui Ren#, Yetian Su, Yuetong Ji, Yaoping Liu, Chunyu Li, Xunrong Li, Yi Zhang, Wei Wang, Qiang Hu, Danxiang Han, Jian Xu*, Bo Ma*. Raman-activated droplet sorting (RADS) for label-free high-throughput screening of microalgal single-cells. Analytical Chemistry, 2017, 89(22): 12569-12577
