Accurate cell characterization and sorting using optical tweezers within the microscopic field
High-quality Single-cell Raman Spectra (SCRS) acquisition – functional information at single-cell resolution
Intact sorted cells are compatible with single-cell sequencing or cultivation
RACS-Seq® is an integrated single-cell analyzer and sorter capable of cell metabolic phenotype profiling, Raman-activated cell sorting, and sample preparation for single-cell genome sequencing and cultivation. Based on single-cell Raman spectroscopy, RACS-Seq® allows direct identification of microbial species and measurement of a wide range of metabolic phenotypes and intercellular heterogeneity in a culture-free manner.
Moreover, in RACS-Seq®, each cell sorted by its metabolic phenotype can be encapsulated in a microdroplet for single-cell DNA/RNA amplification, enabling the construction of high-coverage, low-bias single-cell nucleic acid libraries.
Single-cell Raman Spectra (SCRS) can readily distinguish cell species, and reveal catabolism, anabolism, metabolite-interaction network, stress/drug response, and interaction of cells simultaneously.
Maintain full visibility throughout the process. RACS-Seq® can precisely control the movement trajectory of target cells with optical tweezers, and encapsulate each cell into a microliter-scale microdroplet.
Cells prepared with RACS-Seq® are ideal for single-cell sequencing with reduced MDA amplification bias. The genome coverage of single E. coli cell can reach up to 99.5%,a significant increase over classical approaches (23%).
| Sample Type | Environmental, marine, and soil microbial samples, isolated strains, mammalian cells |
| Cell Size | 0.5-50 μm |
| Sample Requirements |
Optimal loading concentration: 106 to 107 cells/mL Recommended volume: ≥ 10 μL |
| Sorting Environment | Aqueous environment |
| Single-cell Recovery Rate | ≥ 90% |
| Dimensions (W × D × H) |
1166 × 787 × 690 mm |
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