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DCPDigital Colony Picker

  • Highly parallelized microfluidic device for microbial and mammalian cell culture

  • Cell cultivation in a microfluidic chip containing 16,000 physically isolated, picoliter-scale microchambers

  • Growth and functional phenotype monitoring via brightfield and fluorescence imaging

  • AI-powered strain de-duplication

  • Precise, contact-free clone recovery using laser-induced bubble (LIB) technology

  • Fully automated workflow for clone selection and recovery

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Digital Colony Picker (DCP) is a microfluidic device engineered for large-scale, parallel cultivation of single cells followed by automated selection and retrieval of functional clones. It features cultivation in an aqueous environment within each of the 16,000 physically isolated microchambers, real-time phenotypic monitoring during cell growth, and fully automated, AI-powered selection and recovery of clones exhibiting desired growth and functional phenotypes. Compatible with both microbial and mammalian cells, DCP is ideal for applications including strain and cell line development, environmental microbial resource mining, and microbiome research.

Features

  • Cell Cultivation in Independent Microchambers

    Cultivation of single cells in independent microchambers facilitates recovery of slow-growing or low-abundance cells

  • Flexible Reagent Exchange

    Streamlined reagent exchange within chambers enables a wide range of experimental designs

  • High-Efficiency Intelligent Clone Screening

    AI-based image recognition (brightfield and fluorescence) enables automatic, precise targeting of desired microchamber locations, followed by one-click export of selected clones

  • Intuitive Data Visualization and Traceability

    Multiple data visualization options, field-of-view toggling, and high-magnification imaging facilitate precise monitoring and tracking of cultivation chambers

  • Ultra-Low Consumable Usage

    5,000+ single clones (equivalent to 50+ agar plates) can be recovered from each chip, with significant reduction in experimental costs

  • Reduced Contamination Risk

    Non-contact colony recovery and water-in-oil microdroplet collection reduce the need for frequent consumable changes and minimize aerosol contamination

Specifications
Cell Type Bacterial, archaeal, fungal, microalgal, and mammalian cells
Sample Volume Requirement ＞ 20 μL
Fluorescence Excitation Center Wavelength

470 nm (blue) and 530 nm (green)

Number of Microchambers on Chip 16,000
Colony Identifcation Al-powered image recognition (brightfield / fluorescence)
Colony Identifcation Speed
 		1,000 colonies / hour
Collection Plate
 		96-well microtiter plates for PCR or cultivation
Dimensions (W × D × H) 900 × 535 × 425 mm

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