Contact-free colony picking
Physical isolation of single cells
Microdroplet cultivation
Phenotypic monitoring of microdroplet cultivation process (based on brightfield/fluorescence imaging)
Target colony picking with microdroplets
Digital Colony Picker (DCP) enables large-scale, parallelized physical separation of target single cells with specific functions from a genetically heterogeneous cell population. It supports microdroplet-based cultivation, phenotypic monitoring of each microdroplet during the cultivation process, and fully automated selection of droplets containing pure cultures that exhibit desired phenotypes. This instrument can be widely applied to parallelized cultivation of various cell types, as well as the intelligent, automated selection of desired clones.
AI-based image recognition (brightfield/fluorescence) enables automatic and precise targeting of desired chambers, and one-click export of target clones
5,000+ single clones (equivalent to 50+ agar plates) can be recovered from each chip, with significant reduction in experimental costs
Cultivation of single cells in independent chambers culturing effectively minimizes interference from fast growing cells
Streamlined reagent exchange within chambers enables a wide range of experimental designs
Offers multiple data visualization options, field-of-view toggling capability, and high-magnification imaging to observe cultivation chambers
Non-contact colony recovery and water-in-oil microdroplet collection reduce the need for frequent consumable changes and minimize aerosol contamination
| Cell Type | Bacterial, archaeal, and fungal cells; expandable to microalgae, plant, animal, and human cells |
| Sample Volume Requirement | Sample volume ＞ 20 μL |
| Excitation Wavelength |
Blue-green light with center wavelengths of 470 nm and 530 nm |
| Number of Microchambers on Chip | 16,000 microchambers (expandable), pL-volume for each microchamber |
| Colony Identifcation Approach | Al-assisted image recognition (brightfield / fluorescence) |
|
Colony Identifcation Speed |
1,000 single colonies per
hour |
|
Collection Plate |
96-well
microtiter plates for PCR or cultivation |
| Dimensions (W × D × H) |
900 × 535 × 425 mm |
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