Analysis of live cells without labeling
High-throughput, fully automated single-cell analysis and sorting
High-quality Single-cell Raman Spectra (SCRS) acquisition
Minimal impact on biological activity of sorted cells
FlowRACS® is a high-throughput flow cytometer featuring metabolic phenotype-based cell characterization and sorting with single-cell Raman spectroscopy. Inside FlowRACS®, cells are analyzed and sorted without labeling or staining. Using a patented technology, cells are aligned and passed sequentially through a checkpoint, where Raman spectrum of each cell is accurately determined. Live cells with targeted Raman spectra or metabolic functions can then be collected for desired downstream analysis. The throughput of FlowRACS® can reach up to 2,000 cells/min.
Single-cell Raman spectra obtained in FlowRACS® experiments can simultaneously reveal cellular identity and functions including catabolism, anabolism, metabolite-interaction network, stress/drug response, and species interaction. FlowRACS® employs an on-the-fly full Raman spectrum analysis algorithm that interprets and analyzes Raman spectrum in real-time as they are being collected for increased throughput and full automation. The instrument represents the next-generation solution for rapid and low-cost metabolic phenotype profiling, function-based mining of cells and enzymes from nature, and dissection of functional mechanisms of complex biological systems, all at single-cell resolution.
FlowRACS® utilizes Raman spectroscopy without the need for any invasive or potentially harmful labeling such as fluorescent labeling) to identify the metabolic phenotypes of target cells, expanding the scope of its applications.
FlowRACS® allows fastmoving cells to be briefly trapped at a detection point for accurate Raman analysis, maximizing the throughput of cell characterization and sorting.
Experience one-click, fully automated cell sorting with throughput of up to 2000 events/min with minimal human intervention. Benefit from real-time intelligent Raman spectrum analysis following data acquisition, enabling effortless, fully automated cell s
Raman spectrum acquisition and cell sorting are carried out in an aqueous environment, minimizing impact on cell viability and genome integrity.
| Cell Type | Wild-type or engineered bacterial, yeast, and mammalian cells |
| Cell Size | 1-40 μm |
| Sample Loading Concentration | 100-10,000 cell/mL |
| Analysis Rate | 60-3,000 cells/min |
| Sorting Rate | 60-2,000 events/min |
| Dimensions (W × D × H) | 1145 × 770 × 645 mm |
