Effcient identifcation and screening of functional enzyme libraries for functional enzyme resource mining

Home > Applications
Saccharomyces cerevisiae FlowRACS

Highlights

• With single-cell Raman spectroscopy, characteristic peaks correlated with diacylglycerol acyltransferase activity can be identified

• Rapid screening of functional enzyme libraries can be accomplished on FlowRACS®, High-throughput Raman-activated Flow Cytometer

• With FlowRACS®, 3 previously reported strong genes and 2 novel weak genes were obtained in a 10-minute run,compared to months of work using conventional approaches

Abstract

Diacylglycerol acyltransferase (DGAT) is a key enzyme catalyzing the final step of lipid synthesis in human, animals, and plants, and is the only rate-limiting enzyme. Using FlowRACS®, label-free,high-throughput, high-accuracy, non-destructive sorting of DGAT mutant libraries can be achieved at single-cell resolution. In this experiment, from a library of candidate DGAT genes derived from Micrococcus, three previously reported strong genes and two never-reported weak genes were obtained by FlowRACS® in a10-minute run, whereas the screening and characterization of these three strong functional genes with traditional methods would have taken several months. In conclusion, FlowRACS® provides a powerful tool for mining highly efficient enzymes.

Graphical Abstract

Download PDF for more details

Please fill in the required (*) fields to download PDF.

  • First name*
    Last name*
  • Institution/Company Name*
    Title
  • Email*
    Phone number
  • Confirm email*
    Country
  • Area of interest*
  • Comments

l would like to speak with an eCyte Specialist.

l want to stay up-to-date with the latest eCyte news.

By clicking on the "Continue" button below, you are acknowledging and consenting to the collection and use of this information by eCyte, Inc. in accordance with our Terms of Use and Privacy Policy.

Continue

This Website Uses Cookies

We use cookies that are essential for the proper functioning of this website. With your consent, we may also use additional cookies set by eCyte and third-party partners to enhance your browsing experience, enable social media features, and analyze website traffic.

To learn more about how we use cookies, please refer to our Cookie Policy.

By clicking 'Accept', you consent to the use of all cookies, including non-essential ones.

By clicking 'Decline All', only strictly necessary cookies will be used, including one cookie to remember your preference.

By clicking 'Settings', you can customize your cookie preferences.

You can change or withdraw your consent at any time by clicking on 'Cookie Settings' in the website footer.

Settings Accept Decline All
x

About Cookies

Cookies are small text files stored on your browser when you visit a website. Widely used across the web, cookies help websites remember user preferences, improve functionality, and analyze user behavior.

At eCyte, we value your privacy. You have control over which categories of cookies you allow. Use the toggle switches below to manage your preferences. A blue toggle indicates consent for that cookie category.

You can update your preferences anytime by clicking 'Cookie Settings' in the footer of our website. For more information, please refer to our Cookie Policy.

  • Necessary
    Always active

    These cookies are essential for the website to function and cannot be disabled. They support core features such as page navigation, secure access, and service availability. Some are set only in response to actions you take, such as submitting a form. Where personal data is involved, we process it based on our legitimate interest in delivering and securing our services, in accordance with the GDPR.

  • Analytics

    These cookies help us understand how visitors interact with our site. They allow us to count visits, see traffic sources, and identify which pages are most or least popular. This information is collected anonymously and helps us enhance website performance and user experience.

  • Functionality

    These cookies enable enhanced features and personalization, such as remembering your language preference or location. They may be set by us or by third-party providers integrated into our site. Disabling these may impact the functionality of some services.

ACCEPT SAVE SETTINGS